Tinalera also points out that theme is carried by character behavior, and therefore many characters can carry many themes.
But, I'd say that in general you have one central theme, otherwise you run the risk of weakening the story's spine. I'd say the central theme is Tinalera's #1, which is the Frankenstein mythos of science gone wrong. The various characters support that theme, each by having their own take or reaction to it, each seeing it -- like the blind man and the elephant -- from a different perspective. So while they seem like additional themes, I'd say that they are basically sub-themes of the main theme as filtered through each person's viewpoint.